2023 Awards

Omid Madadgar-Master’s student

2023 Dermal Toxicology SS Edgewell Personal Care Student and Postdoctoral Award
Title: “Phosgene Oxime Skin Exposure Causes Oxidative Damage to Biomolecules Including Lipids, Proteins, and DNA in Murine Skin Model”

Andrew Roney-Graduate Student

MSU College of Osteopathic Medicine Research Day
2nd place in the Resident, Fellow, Trainee Poster Presentation competition.

Ebenezar Okoyeocha-Graduate Student

2023 Ocular Toxicology SS Best Poster Award
Title: “Progression of chloropicrin induced ocular injury: clinical and biological effects”

2023 Ocular Toxicology SS Graduate Student and Postdoctoral Fellow Trainee Support Award

Ellen Kim-Undergraduate Student

ASPET travel award to the 2023 Annual Meeting in St. Louis, Missouri
Poster Title: “Cutaneous Exposures to Nitrogen Mustard in C57BL/6 Mice Cause Hematologic Toxicity that could lead to Acute and Long-term Effects”

2022 Awards

Ebenezar Okoyeocha, EITS student training with Dr. Neera Tewari-Singh, received a travel award for his presentation in the 15th Annual CounterACT Network Research Symposium in New Orleans, Louisiana, in June 2022. The symposium was organized by the National Institute of Environental Health Sciences, the National Institute of Allergy and Infectious Diseases and the National Institute of Health Office of the Director. Okoyeocha spoke on, “The Role of Nrf2 Pathway in Chloropicrin Induced Ocular Toxicity in Human Corneal Epithelial Cells and Mice.” (IIT Newsletter, October 2022).

Ellen Kim-Undergraduate Student

Michigan SOT Meeting
Ellen Kim receive second place in the undergraduate poster presentation category for presenting Hematological Consequences of Dermal Exposure to Chemical Threat Agent. Fourth place went to Madeline Godziela for Lung Injury following cutaneous Phosgene Oxime Exposure in SKH-1 Hairless Mice.

Maddie Godziela – Undergraduate Student

Fall 2022 Meeting of the Michigan Chapter of the Society of Toxicology
Fourth place for Outstanding Poster, Lung Injury following cutaneous Phosgene Oxime Exposure in SKH-1 Hairless Mice.

2021 Awards

Dr. Neera Tewari-Singh

Society of Toxicology-Ocular Toxicology Specialty Section Ocular Toxicology I2 Award (Innovation and Impact) Sponsored by Biomere. This award recognizes innovations and/or scientific advances that Impact the field of ocular toxicology based on papers published.

Dr. Satyendra Singh

Society of Toxicology-Dermal Toxicology Specialty Section, Stratacor Postdoctoral Award. Awarded to Postdoctoral candidates for their contribution to skin-related research.

Dr. Swati Sharma

Society of Toxicology-Dermal Toxicology Specialty Section Postdoctoral Award Sponsored by Charles River. Awarded for skin-related toxicology research.

Ryan Kerin

American Physician Scientists Association Fall Research Conference. Awarded 1st Place in Best Poster Competition.

Publications

Mishra N, Kant R, Goswami DG, Petrash JM, Agarwal C, Tewari-Singh N, Agarwal R. Metabolomics for identifying pathways involved in vesicating agent lewisite- induced corneal injury. Exp Eye Res. 2023 Nov;236:109672. doi: 10.1016/j.exer.2023.109672. Epub 2023 Oct 4. PMID: 37797797.

Goswami DG, Singh SK, Okoyeocha EO, Roney AK, Madadgar O, Tuttle R, Sosna W, Anantharam P, Croutch CR, Agarwal R, Tewari-Singh N. Dermal Exposure to Vesicating Nettle Agent Phosgene Oxime: Clinically Relevant Biomarkers and Skin Injury Progression in Murine Models. J Pharmacol Exp Ther. 2023 Aug 31:JPET- AR-2023-001718. doi: 10.1124/jpet.123.001718. Epub ahead of print. PMID: 37652710.

Mishra N, Kant R, Kandhari K, Ammar DA, Tewari-Singh N, Pantcheva MB, Petrash JM, Agarwal C, Agarwal R. Nitrogen mustard-induced ex vivo human cornea injury model and therapeutic intervention by dexamethasone. J Pharmacol Exp Ther. 2023 Jul 20:JPET-AR-2023-001760. doi: 10.1124/jpet.123.001760. Epub ahead of print. PMID: 37474260.

Mishra N, Kant R, Kandhari K, Tewari-Singh N, Anantharam P, Croutch CR, Pantcheva MB, Petrash JM, Araj H, Agarwal C, Agarwal R. Establishing a dexamethasone treatment regimen to alleviate sulfur mustard-induced corneal injuries in a rabbit model. J Pharmacol Exp Ther. 2023 Jun 14:JPET- AR-2023-001680. doi: 10.1124/jpet.123.001680. Epub ahead of print. PMID: 37316330.

Ebenezar OO, Roney A, Goswami DG, Petrash JM, Sledge D, Komáromy AM, Liby KT, Tewari-Singh N. Ocular injury progression and cornea histopathology from chloropicrin vapor exposure: Relevant clinical biomarkers in mice. Exp Eye Res. 2023 May;230:109440. doi: 10.1016/j.exer.2023.109440. Epub 2023 Mar 17. PMID: 36933694.

Cruz-Hernandez A, Roney A, Goswami DG, Tewari-Singh N, Brown JM. A review of chemical warfare agents linked to respiratory and neurological effects experienced in Gulf War Illness. Inhal Toxicol. 2022;34(13-14):412-432. doi: 10.1080/08958378.2022.2147257. Epub 2022 Nov 17. PMID: 36394251; PMCID: PMC9832991.

Goswami DG, Mishra N, Kant R, Agarwal C, Ammar DA, Petrash JM, Tewari-Singh N, Agarwal R. Effect of dexamethasone treatment at variable therapeutic windows in reversing nitrogen mustard-induced corneal injuries in rabbit ocular in vivo model. Toxicol Appl Pharmacol. 2022 Feb 15;437:115904. doi: 10.1016/j.taap.2022.115904. Epub 2022 Jan 30. PMID: 35108561; PMCID: PMC8849585.

Goswami DG, Mishra N, Kant R, Agarwal C, Croutch CR, Enzenauer RW, Petrash MJ, Tewari-Singh N, Agarwal R. Pathophysiology and inflammatory biomarkers of sulfur mustard-induced corneal injury in rabbits. PLoS One. 2021 Oct 12;16(10):e0258503. doi: 10.1371/journal.pone.0258503. PMID: 34637469; PMCID: PMC8509852.

Cruz-Hernandez A, Roney A, Goswami DG, Tewari-Singh N, Brown JM. A review of chemical warfare agents linked to respiratory and neurological effects experienced in Gulf War Illness. Inhal Toxicol. 2022;34(13-14):412-432. doi:10.1080/08958378.2022.2147257. Epub 2022 Nov 17. PMID: 36394251; PMCID:PMC9832991.

Cruz-Hernandez A, Mendoza RP, Nguyen K, Harder A, Evans CM, Bauer AK, Tewari- Singh N, Brown JM. Mast cells promote nitrogen mustard mediated toxicity in the lung associated with proinflammatory cytokine and bioactive lipid mediator production. Toxicol Sci. 2021 Aug 28:kfab107. doi: 10.1093/toxsci/kfab107. Epub ahead of print. PMID: 34453837.

Singh SK, Goswami DG, Wright HN, Kant R, Ali IA, Braucher LN, Klein JA, Godziela MG, Ammar DA, Pate KM, Tewari-Singh N. Effect of supersaturated oxygen emulsion treatment on chloropicrin-induced chemical injury in ex vivo rabbit cornea. Toxicol Lett. 2021 Oct 1;349:124-133. doi: 10.1016/j.toxlet.2021.06.015. Epub 2021 Jun 18. PMID: 34153409.

Casillas RP, Tewari-Singh N, Gray JP. Special issue: emerging chemical terrorism threats. Toxicol Mech Methods. 2021 May;31(4):239-241. doi: 10.1080/15376516.2021.1904472. PMID: 33730980.

Singh SK, Klein JA, Wright HN, Tewari-Singh N. Phosgene oxime: a highly toxic urticant and emerging chemical threat. Toxicol Mech Methods. 2021 May;31(4):288-292. doi: 10.1080/15376516.2020.1861670. Epub 2020 Dec 22. PMID: 33297803; PMCID: PMC8087642.

Goswami DG, Kant R, Ammar DA, Kumar D, Enzenauer RW, Petrash JM, Tewari-Singh N, Agarwal R. Acute corneal injury in rabbits following nitrogen mustard ocular exposure. Exp Mol Pathol. 2019 Oct;110:104275. doi: 10.1016/j.yexmp.2019.104275. Epub 2019 Jun 21. PMID: 31233733; PMCID: PMC6754274.

Pate KM, Goswami DG, Lake M, Lake S, Kant R, Ammar D, Tewari-Singh N. A Supersaturated Oxygen Emulsion for the Topical Treatment of Ocular Trauma. Mil Med. 2020 Mar 2;185(3-4):e466-e472. doi: 10.1093/milmed/usz337. PMID: 31865377.

Goswami DG, Kant R, Ammar DA, Agarwal C, Gomez J, Agarwal R, Saba LM, Fritz KS, Tewari-Singh N. Toxic consequences and oxidative protein carbonylation from chloropicrin exposure in human corneal epithelial cells. Toxicol Lett. 2020 Apr 1;322:1-11. doi: 10.1016/j.toxlet.2019.12.023. Epub 2019 Dec 26. PMID: 31884112.

Goswami DG, Tewari-Singh N, Agarwal R. Corneal toxicity induced by vesicating agents and effective treatment options. Ann N Y Acad Sci. 2016 Jun;1374(1):193-201. doi: 10.1111/nyas.13121. Epub 2016 Jun 21. PMID: 27327041; PMCID: PMC4940229.

Goswami DG, Agarwal R, Tewari-Singh N. Phosgene oxime: Injury and associated mechanisms compared to vesicating agents sulfur mustard and lewisite. Toxicol Lett. 2018 Sep 1;293:112-119. doi: 10.1016/j.toxlet.2017.11.011. Epub 2017 Nov 12. PMID: 29141200; PMCID: PMC5949076.

Tewari-Singh N, Agarwal R. Mustard vesicating agent-induced toxicity in the skin tissue and silibinin as a potential countermeasure. Ann N Y Acad Sci. 2016 Jun;1374(1):184-92. doi: 10.1111/nyas.13099. Epub 2016 Jun 21. PMID: 27326543; PMCID: PMC4940265.

Tewari-Singh N, Croutch CR, Tuttle R, Goswami DG, Kant R, Peters E, Culley T, Ammar DA, Enzenauer RW, Petrash JM, Casillas RP, Agarwal R. Clinical progression of ocular injury following arsenical vesicant lewisite exposure. Cutan Ocul Toxicol. 2016 Dec;35(4):319-28. doi: 10.3109/15569527.2015.1127255. Epub 2016 Mar 22. PMID: 27002633; PMCID: PMC5082841.

Tewari-Singh N, Goswami DG, Kant R, Ammar DA, Kumar D, Enzenauer RW, Casillas RP, Croutch CR, Petrash JM, Agarwal R. Histopathological and Molecular Changes in the Rabbit Cornea From Arsenical Vesicant Lewisite Exposure. Toxicol Sci. 2017 Dec 1;160(2):420-428. doi: 10.1093/toxsci/kfx198. PMID: 28973427; PMCID: PMC5837587.

Jain AK, Tewari-Singh N, Inturi S, Orlicky DJ, White CW, Agarwal R. Myeloperoxidase deficiency attenuates nitrogen mustard-induced skin injuries. Toxicology. 2014 Jun 5;320:25-33. doi: 10.1016/j.tox.2014.02.013. Epub 2014 Mar 12. PMID: 24631667; PMCID: PMC4019690.

Tewari-Singh N, Goswami DG, Kant R, Croutch CR, Casillas RP, Orlicky DJ, Agarwal R. Cutaneous exposure to vesicant phosgene oxime: Acute effects on the skin and systemic toxicity. Toxicol Appl Pharmacol. 2017 Feb 15;317:25-32. doi: 10.1016/j.taap.2017.01.003. Epub 2017 Jan 11. PMID: 28087322.

Tewari-Singh N, Agarwal C, Huang J, Day BJ, White CW, Agarwal R. Efficacy of glutathione in ameliorating sulfur mustard analog-induced toxicity in cultured skin epidermal cells and in SKH-1 mouse skin in vivo. J Pharmacol Exp Ther. 2011 Feb;336(2):450-9. doi: 10.1124/jpet.110.173708. Epub 2010 Oct 25. PMID: 20974699; PMCID: PMC3033716.

Antunes MS, Ha SB, Tewari-Singh N, Morey KJ, Trofka AM, Kugrens P, Deyholos M, Medford JI. A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity. Plant Biotechnol J. 2006 Nov;4(6):605-22. doi: 10.1111/j.1467-7652.2006.00205.x. PMID: 17309732.

Goswami DG, Kant R, Tewari-Singh N, Agarwal R. Efficacy of anti- inflammatory, antibiotic and pleiotropic agents in reversing nitrogen mustard- induced injury in ex vivo cultured rabbit cornea. Toxicol Lett. 2018 Sep 1;293:127-132. doi: 10.1016/j.toxlet.2017.11.026. Epub 2017 Nov 23. PMID: 29174984.

Goswami DG, Kumar D, Tewari-Singh N, Orlicky DJ, Jain AK, Kant R, Rancourt RC, Dhar D, Inturi S, Agarwal C, White CW, Agarwal R. Topical nitrogen mustard exposure causes systemic toxic effects in mice. Exp Toxicol Pathol. 2015 Feb;67(2):161-70. doi: 10.1016/j.etp.2014.11.006. Epub 2014 Dec 4. PMID: 25481215; PMCID: PMC4308427.

Jain AK, Tewari-Singh N, Inturi S, Kumar D, Orlicky DJ, Agarwal C, White CW, Agarwal R. Flavanone silibinin treatment attenuates nitrogen mustard-induced toxic effects in mouse skin. Toxicol Appl Pharmacol. 2015 May 15;285(1):71-8. doi: 10.1016/j.taap.2015.03.009. Epub 2015 Mar 16. PMID: 25791923; PMCID: PMC4410080.

Casillas RP, Tewari-Singh N, Gray JP. Special issue: emerging chemical terrorism threats. Toxicol Mech Methods. 2021 May;31(4):239-241. doi: 10.1080/15376516.2021.1904472. PMID: 33730980.

Goswami DG, Tewari-Singh N, Dhar D, Kumar D, Agarwal C, Ammar DA, Kant R, Enzenauer RW, Petrash JM, Agarwal R. Nitrogen Mustard-Induced Corneal Injury Involves DNA Damage and Pathways Related to Inflammation, Epithelial-Stromal Separation, and Neovascularization. Cornea. 2016 Feb;35(2):257-66. doi: 10.1097/ICO.0000000000000685. PMID: 26555588; PMCID: PMC4706783.

Tewari-Singh N, Jain AK, Orlicky DJ, White CW, Agarwal R. Cutaneous injury- related structural changes and their progression following topical nitrogen mustard exposure in hairless and haired mice. PLoS One. 2014 Jan 8;9(1):e85402. doi: 10.1371/journal.pone.0085402. PMID: 24416404; PMCID: PMC3885697.

Tewari-Singh N, Jain AK, Inturi S, Ammar DA, Agarwal C, Tyagi P, Kompella UB, Enzenauer RW, Petrash JM, Agarwal R. Silibinin, dexamethasone, and doxycycline as potential therapeutic agents for treating vesicant-inflicted ocular injuries. Toxicol Appl Pharmacol. 2012 Oct 1;264(1):23-31. doi: 10.1016/j.taap.2012.07.014. Epub 2012 Jul 24. PMID: 22841772; PMCID: PMC3928638.

Inturi S, Tewari-Singh N, Jain AK, Roy S, White CW, Agarwal R. Absence of a p53 allele delays nitrogen mustard-induced early apoptosis and inflammation of murine skin. Toxicology. 2013 Sep 15;311(3):184-90. doi: 10.1016/j.tox.2013.06.013. Epub 2013 Jul 8. PMID: 23845566; PMCID: PMC3808076.

Tewari-Singh N, Inturi S, Jain AK, Agarwal C, Orlicky DJ, White CW, Agarwal R, Day BJ. Catalytic antioxidant AEOL 10150 treatment ameliorates sulfur mustard analog 2-chloroethyl ethyl sulfide-associated cutaneous toxic effects. Free Radic Biol Med. 2014 Jul;72:285-95. doi: 10.1016/j.freeradbiomed.2014.04.022. Epub 2014 May 9. PMID: 24815113; PMCID: PMC4080896.

Jain AK, Tewari-Singh N, Inturi S, Orlicky DJ, White CW, Agarwal R. Histopathological and immunohistochemical evaluation of nitrogen mustard-induced cutaneous effects in SKH-1 hairless and C57BL/6 mice. Exp Toxicol Pathol. 2014 Mar;66(2-3):129-38. doi: 10.1016/j.etp.2013.11.005. Epub 2013 Dec 25. PMID: 24373750; PMCID: PMC3923453.

Inturi S, Tewari-Singh N, Agarwal C, White CW, Agarwal R. Activation of DNA damage repair pathways in response to nitrogen mustard-induced DNA damage and toxicity in skin keratinocytes. Mutat Res. 2014 May-Jun;763-764:53-63. doi: 10.1016/j.mrfmmm.2014.04.002. Epub 2014 Apr 13. PMID: 24732344; PMCID: PMC4199943.

Antunes MS, Morey KJ, Tewari-Singh N, Bowen TA, Smith JJ, Webb CT, Hellinga HW, Medford JI. Engineering key components in a synthetic eukaryotic signal transduction pathway. Mol Syst Biol. 2009;5:270. doi: 10.1038/msb.2009.28. Epub 2009 May 19. PMID: 19455134; PMCID: PMC2694678.

Tewari-Singh N, Jain AK, Inturi S, White CW, Agarwal R. Clinically-relevant cutaneous lesions by nitrogen mustard: useful biomarkers of vesicants skin injury in SKH-1 hairless and C57BL/6 mice. PLoS One. 2013 Jun 24;8(6):e67557. doi: 10.1371/journal.pone.0067557. PMID: 23826320; PMCID: PMC3691145.

Inturi S, Tewari-Singh N, Gu M, Shrotriya S, Gomez J, Agarwal C, White CW, Agarwal R. Mechanisms of sulfur mustard analog 2-chloroethyl ethyl sulfide- induced DNA damage in skin epidermal cells and fibroblasts. Free Radic Biol Med. 2011 Dec 15;51(12):2272-80. doi: 10.1016/j.freeradbiomed.2011.08.020. Epub 2011 Aug 26. PMID: 21920433; PMCID: PMC3662483.

Jain AK, Tewari-Singh N, Orlicky DJ, White CW, Agarwal R. 2-Chloroethyl ethyl sulfide causes microvesication and inflammation-related histopathological changes in male hairless mouse skin. Toxicology. 2011 Apr 11;282(3):129-38. doi: 10.1016/j.tox.2011.01.021. Epub 2011 Feb 2. PMID: 21295104; PMCID: PMC3085827.

Gao X, Belmadani S, Picchi A, Xu X, Potter BJ, Tewari-Singh N, Capobianco S, Chilian WM, Zhang C. Tumor necrosis factor-alpha induces endothelial dysfunction in Lepr(db) mice. Circulation. 2007 Jan 16;115(2):245-54. doi: 10.1161/CIRCULATIONAHA.106.650671. Epub 2007 Jan 2. PMID: 17200442.

Kumar D, Tewari-Singh N, Agarwal C, Jain AK, Inturi S, Kant R, White CW, Agarwal R. Nitrogen mustard exposure of murine skin induces DNA damage, oxidative stress and activation of MAPK/Akt-AP1 pathway leading to induction of inflammatory and proteolytic mediators. Toxicol Lett. 2015 Jun 15;235(3):161-71. doi: 10.1016/j.toxlet.2015.04.006. Epub 2015 Apr 16. PMID: 25891025; PMCID: PMC4430414.

Pal A, Tewari-Singh N, Gu M, Agarwal C, Huang J, Day BJ, White CW, Agarwal R. Sulfur mustard analog induces oxidative stress and activates signaling cascades in the skin of SKH-1 hairless mice. Free Radic Biol Med. 2009 Dec 1;47(11):1640-51. doi: 10.1016/j.freeradbiomed.2009.09.011. Epub 2009 Sep 15. PMID: 19761830; PMCID: PMC2801552.

Jain AK, Tewari-Singh N, Gu M, Inturi S, White CW, Agarwal R. Sulfur mustard analog, 2-chloroethyl ethyl sulfide-induced skin injury involves DNA damage and induction of inflammatory mediators, in part via oxidative stress, in SKH-1 hairless mouse skin. Toxicol Lett. 2011 Sep 10;205(3):293-301. doi: 10.1016/j.toxlet.2011.06.019. Epub 2011 Jun 21. PMID: 21722719; PMCID: PMC3207490.

Tewari-Singh N, Gu M, Agarwal C, White CW, Agarwal R. Biological and molecular mechanisms of sulfur mustard analogue-induced toxicity in JB6 and HaCaT cells: possible role of ataxia telangiectasia-mutated/ataxia telangiectasia-Rad3-related cell cycle checkpoint pathway. Chem Res Toxicol. 2010 Jun 21;23(6):1034-44. doi: 10.1021/tx100038b. PMID: 20469912; PMCID: PMC2891139.

Tewari-Singh N, Jain AK, Inturi S, Agarwal C, White CW, Agarwal R. Silibinin attenuates sulfur mustard analog-induced skin injury by targeting multiple pathways connecting oxidative stress and inflammation. PLoS One. 2012;7(9):e46149. doi: 10.1371/journal.pone.0046149. Epub 2012 Sep 27. PMID: 23029417; PMCID: PMC3459894.

Tewari-Singh N, Rana S, Gu M, Pal A, Orlicky DJ, White CW, Agarwal R. Inflammatory biomarkers of sulfur mustard analog 2-chloroethyl ethyl sulfide- induced skin injury in SKH-1 hairless mice. Toxicol Sci. 2009 Mar;108(1):194-206. doi: 10.1093/toxsci/kfn261. Epub 2008 Dec 15. PMID: 19075041; PMCID: PMC2644400.

Tewari-Singh N, Sen J, Kiesecker H, Reddy VS, Jacobsen HJ, Guha-Mukherjee S. Use of a herbicide or lysine plus threonine for non-antibiotic selection of transgenic chickpea. Plant Cell Rep. 2004 Mar;22(8):576-83. doi: 10.1007/s00299-003-0730-6. Epub 2004 Jan 29. PMID: 14749891.

Singh SK, Goswam DG, Wright HN, Kant R, Ali IA, Braucher LN, Klein JA, Godziela MG, Ammar DA, Pate KM, Tewari-Singh N. Effect of supersaturated oxygen emulsion treatment on chloropicrin-induced chemical injury in ex vivo rabbit cornea. Toxicol Lett. 2021 Jun 18:S0378-4274(21)00167-3. doi: 10.1016/j.toxlet.2021.06.015. Epub ahead of print. PMID: 34153409.